The Stoichiometry of Ribosomal Translocation
نویسندگان
چکیده
منابع مشابه
The stoichiometry of ribosomal translocation.
Fusidic acid does not inhibit the translocation of N-acetylphenylalanyl-tRNA (N-acetyl-Phe-tRNA), bound nonenzymitally to the ribosomal A-site, when the amount of ribosomes in the reaction mixture is not sufficient to trap all the available elongation factor G (EF-G) in the form of GDP ‘EF-G .fusidic acid .ribosome complexes. Moreover, since only those ribosomes in the reaction mixture that are...
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1. CC-BY-NC-ND 4.0 International license peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was not. Understanding the regulation and structure of the eukaryotic ribosome is essential to understanding protein synthesis and its deregulation in disease. While ribosomes are believed to have a fixed stoichiometry among their core ribo-som...
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Understanding the regulation and structure of ribosomes is essential to understanding protein synthesis and its dysregulation in disease. While ribosomes are believed to have a fixed stoichiometry among their core ribosomal proteins (RPs), some experiments suggest a more variable composition. Testing such variability requires direct and precise quantification of RPs. We used mass spectrometry t...
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Recent results from cryo-electron microscopy have shown that substantial structural rearrangements in both elongation factor EF-G and the ribosome occur during tRNA translocation. The observed sites of interaction between EF-G and the ribosome are consistent with molecular mimicry models for EF-G function.
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machinery are complex, and an article in Journal of Biology [1] suggests that the way that ribosomes operate is more complex than had previously been suspected (see ‘The bottom line’ box for a summary of the work). Many factors assist the ribosome with the business of knitting amino acids together according to the instructions within mRNAs, so as to form proteins. Up until now it had been assum...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)43140-2